Journal: Oncotarget

Article Title: Effect of TIMPs and their minimally engineered variants in blocking invasion and migration of brain cancer cells.

doi: 10.18632/oncotarget.28691

Figure Lengend Snippet: Figure 1: Effect of minimal TIMP variant (mTC1) on the proliferation of GBM cell lines (T98G, A172) and HeLa cell line. The experiment was performed 24 hours after adding the mTC1 peptide to the GBM cell lines. The MTT assay was performed for T98G and A172. Hela cell line was used as a control. Bar graphs represent the mean ± SEM of 4 different MTT experiments. Statistical analysis was performed using One-way ANOVA. *P < 0.05.

Article Snippet: A172 glioma cell line was purchased from ATCC (CRL-1620TM, ATCC, Manassas, VA, USA).

Techniques: Variant Assay, MTT Assay, Control

Journal: Oncotarget

Article Title: Effect of TIMPs and their minimally engineered variants in blocking invasion and migration of brain cancer cells.

doi: 10.18632/oncotarget.28691

Figure Lengend Snippet: Figure 2: Cellular uptake of minimal TIMP variants in the T98G and A172 cell lines. Fluorescence images of T98G and A172 cell lines after incubation with FAM-labeled peptides, including mTC1, and CPP-mTC1 for 4 h. The scale bars represent 40 µm. The images are an overlay of the green and blue filters. Green, FAM-labeled peptide; blue, Hoechst-stained cell nuclei.

Article Snippet: A172 glioma cell line was purchased from ATCC (CRL-1620TM, ATCC, Manassas, VA, USA).

Techniques: Fluorescence, Incubation, Labeling, Staining

Journal: Oncotarget

Article Title: Effect of TIMPs and their minimally engineered variants in blocking invasion and migration of brain cancer cells.

doi: 10.18632/oncotarget.28691

Figure Lengend Snippet: Figure 4: Effect of TIMPs and minimal TIMPs variants on migration of the A172 cell line. (A) Representative images of migration A172 in the presence and absence of TIMPs and minimal TIMP variants. The cells were visualized by light microscopy using a 10X magnification lens at 0, 12 h, and 18 h, after adding 1.5 µM of TIMPs (TIMP-1, TIMP-3) and minimal TIMPs variants (mTC1, mTC3) (B) Calculated migration rate of T98G cell line at concentrations of 0.5 and 1.5 µM of TIMPs (TIMP-1, TIMP-3) and minimal TIMPs variants (mTC1, mTC3). The created wounds were analyzed using ImageJ software and wound closure percentage was calculated (The data were normalized to control-untreated cells). The experiments were performed in duplicate; means and standard error are shown. ****p < 0.0001, **P < 0.01, *P < 0.05 as determined by One-way ANOVA comparing inhibition in the presence of the various inhibitors versus the untreated control.

Article Snippet: A172 glioma cell line was purchased from ATCC (CRL-1620TM, ATCC, Manassas, VA, USA).

Techniques: Migration, Light Microscopy, Software, Control, Inhibition

Journal: Oncotarget

Article Title: Effect of TIMPs and their minimally engineered variants in blocking invasion and migration of brain cancer cells.

doi: 10.18632/oncotarget.28691

Figure Lengend Snippet: Figure 6: Effect of TIMPs and minimal TIMP variants on the invasion of A172 cell line. (A) Representative image of invasion A172 cell line in the presence and absence of TIMP-1, TIMP-3, mTC1, and mTC3. The cells were fixed in 100% methanol, stained with 2% crystal violet, and then visualized by light microscopy using an 10X magnification lens. (B) The percentage of invaded cells was counted using the ImageJ software and normalized to the count of untreated cells. The statistical significance was obtained using One-way ANOVA. ****p < 0.0001.

Article Snippet: A172 glioma cell line was purchased from ATCC (CRL-1620TM, ATCC, Manassas, VA, USA).

Techniques: Staining, Light Microscopy, Software